Acquapendente is located at the northern end of Lazio where the Paglia river divides Colli Volsini from Monte Amiata, near the border of Tuscany and Umbria. It is 10 kilometers north of Bolsena lake, a few steps from the Monte Rufeno Nature Reserve and is crossed by the Via Cassia. Acquapendente was the northern entrance to the Papal States for pilgrims on their way to the Jubilee, the path runs through it from north to south, dividing the old town and the valley that lies in two parts. Over the centuries, this route has taken on different names: “Via Cassia” in Roman times, “Via Francigena” throughout the Middle Ages, then “Via Romea” at the time of the great pilgrimages.

At the entrance you find the medieval tower called Julia de Jacopo, the Door of the ancient wall and the Cathedral of Santo Sepolcro, built during the eleventh century at the behest of Matilda of Westphalia. The crypt of the Cathedral dates from the second half of the tenth century; It consists of nine aisles with 22 columns with rich capitals. It was built on the primitive shrine of the Holy Sepulchre which is in the center, covered by a pyramid with a rectangular base. Tradition says that is preserves a relic wet with the blood of Christ.