Casale Galli is located in Trevinano, a hill town most likely of Etruscan origin, in the most northern area of the Lazio region, on the border with Tuscany and Umbria. The villa is located halfway between Rome and Florence; both cities can be reached via the A1 motorway in less than two hours by car.

The nearest airports are in Perugia (80 km), Siena (120 km), Florence (160 km) and Rome (170 km).

The nearest railway stations are in Fabro (20 km), Chiusi (30km) and Orvieto (30 km).